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phospho smad1 5 8  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phospho smad1 5 8
    Phospho Smad1 5 8, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 762 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+smad1/Phospho-Smad1+(Ser463%2F465)%2F+Smad5+(Ser463%2F465)%2F+Smad9+(Ser465%2F467)+Rabbit+mAb/pmc13015696-66-67-82
    Average 96 stars, based on 762 article reviews
    phospho smad1 5 8 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Saline:

    Article Title: High Rate Triggers Increased Atrial Release of BMP10, A Biomarker for Atrial Fibrillation and Stroke, and BMP10 Affects Ventricular Cardiomyocytes
    Article Snippet: Blood samples were immediately processed, stored at −80 °C, and analyzed centrally as reported before, using a high-throughput, high-precision assay (Roche Diagnostics, Penzberg, Germany)., For clinical characteristics of patients, see Table S1 . .. Antibodies were directed against SMAD1 (1:1000 in 5% milk/Tris-Buffered Saline with Tween 20, CST no. 9743), phospho-SMAD1 (pSer463/465)/SMAD5 (Ser463/465)/SMAD-9 (Ser465/467, 1:1000 in 5% milk/Tris-Buffered Saline with Tween 20, CST no. 13820), and GAPDH as loading control (1:2000 in 5% milk/Tris-Buffered Saline with Tween 20, HyTest no. 564). .. Effects of BMP10 on vEHT contractility were assessed by both acute (30 min) and longer-term (10 days) exposure of vEHT to recombinant human BMP10 (rhBMP10; 2926-BP-025, R&D Systems, Minneapolis) dissolved in water containing 0.1% BSA and 4 mmol/L HCl (vehicle).

    Control:

    Article Title: High Rate Triggers Increased Atrial Release of BMP10, A Biomarker for Atrial Fibrillation and Stroke, and BMP10 Affects Ventricular Cardiomyocytes
    Article Snippet: Blood samples were immediately processed, stored at −80 °C, and analyzed centrally as reported before, using a high-throughput, high-precision assay (Roche Diagnostics, Penzberg, Germany)., For clinical characteristics of patients, see Table S1 . .. Antibodies were directed against SMAD1 (1:1000 in 5% milk/Tris-Buffered Saline with Tween 20, CST no. 9743), phospho-SMAD1 (pSer463/465)/SMAD5 (Ser463/465)/SMAD-9 (Ser465/467, 1:1000 in 5% milk/Tris-Buffered Saline with Tween 20, CST no. 13820), and GAPDH as loading control (1:2000 in 5% milk/Tris-Buffered Saline with Tween 20, HyTest no. 564). .. Effects of BMP10 on vEHT contractility were assessed by both acute (30 min) and longer-term (10 days) exposure of vEHT to recombinant human BMP10 (rhBMP10; 2926-BP-025, R&D Systems, Minneapolis) dissolved in water containing 0.1% BSA and 4 mmol/L HCl (vehicle).

    Article Title: High Rate Triggers Increased Atrial Release of BMP10, A Biomarker for Atrial Fibrillation and Stroke, and BMP10 Affects Ventricular Cardiomyocytes
    Article Snippet: Blood samples were immediately processed, stored at −80 °C, and analyzed centrally as reported before, using a high-throughput, high-precision assay (Roche Diagnostics, Penzberg, Germany).6,17 For clinical characteristics of patients, see Table S1. .. Antibodies were directed against SMAD1 (1:1000 in 5% milk/TBST, CST no. 9743), phospho-SMAD1 (pSer463/465)/SMAD5 (Ser463/465)/SMAD-9 (Ser465/467, 1:1000 in 5% milk/TBST, CST no. 13820), and GAPDH as loading control (1:2000 in 5% milk/TBST, HyTest no. 564). .. Effects of BMP10 on vEHT contractility were assessed by both acute (30 min) and longer-term (10 days) exposure of vEHT to recombinant human BMP10 (rhBMP10; 2926-BP-025, R&D Systems, Minneapolis) dissolved in water containing 0.1% BSA and 4 mmol/L HCl (vehicle).

    Membrane:

    Article Title: Deciphering the functional association of novel variants of BMP7 in isolated congenital heart disease by integrating in vitro and in silico approaches
    Article Snippet: .. Membrane was blocked in 5% non-fat dry milk in 1X TBST followed by overnight incubation with phospho-SMAD1/5 primary antibody (CST, USA) at 4°C subsequently washed and probed with HRP conjugated goat anti-Rabbit IgG secondary antibody (Genei, Germany) for 2 hrs at RT. .. Finally, the expression of phospho-SMAD1/5 was detected with chemiluminescence detection kit (GE health Care, USA) using chemiDoc (Amersham TM Imager 680, Japan) following thorough washing (5 times, 5 min each in 1X TBST).

    Article Title: Implication of novel variants of BMP2 in isolated congenital heart disease: Functional characterization by in silico and invitro approaches
    Article Snippet: .. The blocked membrane was incubated with phospho-SMAD1/5 primary antibody (CST, USA) at 4°C overnight followed by washing with 1X TBST (3 times) and then incubated with HRP conjugated goat anti-rabbit IgG antibody (Genei, Germany) for 2 h at RT. .. The membrane was washed with 1X TBST (5 times) and chemiluminescence detection was performed using an ECL detection kit (GE health Care, US) using chemiDoc (Amersham TM Imager 680, Japan).

    Article Title: Functionally significant, novel variants of BMP4 are associated with isolated congenital heart disease
    Article Snippet: 25μg of reducing samples were denatured for 5 min at 95°C in Laemmeli’s loading buffer and lysates were resolved on 8% SDS polyacrylamide gel followed by transferring the proteins to PVDF membrane (Bio-Rad Laboratories Inc, CA, USA). .. After blocking the membrane in 5% non-fat dry milk in 1X TBST for 2 hours at RT, it was incubated with phospho-SMAD1/5 primary antibody (CST, USA) at 4°C for overnight followed by washing with 1X TBST (3 times) and then incubated with HRP conjugated goat anti-Rabbit IgG antibody (Genei, Germany) for 2 hours at RT. .. The membrane was washed with 1X TBST (5 times) and chemiluminescence detection was carried out with ECL detection kit (GE health Care, US) using chemiDoc (Amersham TM Imager 680, Japan).

    Incubation:

    Article Title: Deciphering the functional association of novel variants of BMP7 in isolated congenital heart disease by integrating in vitro and in silico approaches
    Article Snippet: .. Membrane was blocked in 5% non-fat dry milk in 1X TBST followed by overnight incubation with phospho-SMAD1/5 primary antibody (CST, USA) at 4°C subsequently washed and probed with HRP conjugated goat anti-Rabbit IgG secondary antibody (Genei, Germany) for 2 hrs at RT. .. Finally, the expression of phospho-SMAD1/5 was detected with chemiluminescence detection kit (GE health Care, USA) using chemiDoc (Amersham TM Imager 680, Japan) following thorough washing (5 times, 5 min each in 1X TBST).

    Article Title: Implication of novel variants of BMP2 in isolated congenital heart disease: Functional characterization by in silico and invitro approaches
    Article Snippet: .. The blocked membrane was incubated with phospho-SMAD1/5 primary antibody (CST, USA) at 4°C overnight followed by washing with 1X TBST (3 times) and then incubated with HRP conjugated goat anti-rabbit IgG antibody (Genei, Germany) for 2 h at RT. .. The membrane was washed with 1X TBST (5 times) and chemiluminescence detection was performed using an ECL detection kit (GE health Care, US) using chemiDoc (Amersham TM Imager 680, Japan).

    Article Title: Functionally significant, novel variants of BMP4 are associated with isolated congenital heart disease
    Article Snippet: 25μg of reducing samples were denatured for 5 min at 95°C in Laemmeli’s loading buffer and lysates were resolved on 8% SDS polyacrylamide gel followed by transferring the proteins to PVDF membrane (Bio-Rad Laboratories Inc, CA, USA). .. After blocking the membrane in 5% non-fat dry milk in 1X TBST for 2 hours at RT, it was incubated with phospho-SMAD1/5 primary antibody (CST, USA) at 4°C for overnight followed by washing with 1X TBST (3 times) and then incubated with HRP conjugated goat anti-Rabbit IgG antibody (Genei, Germany) for 2 hours at RT. .. The membrane was washed with 1X TBST (5 times) and chemiluminescence detection was carried out with ECL detection kit (GE health Care, US) using chemiDoc (Amersham TM Imager 680, Japan).

    Blocking Assay:

    Article Title: Functionally significant, novel variants of BMP4 are associated with isolated congenital heart disease
    Article Snippet: 25μg of reducing samples were denatured for 5 min at 95°C in Laemmeli’s loading buffer and lysates were resolved on 8% SDS polyacrylamide gel followed by transferring the proteins to PVDF membrane (Bio-Rad Laboratories Inc, CA, USA). .. After blocking the membrane in 5% non-fat dry milk in 1X TBST for 2 hours at RT, it was incubated with phospho-SMAD1/5 primary antibody (CST, USA) at 4°C for overnight followed by washing with 1X TBST (3 times) and then incubated with HRP conjugated goat anti-Rabbit IgG antibody (Genei, Germany) for 2 hours at RT. .. The membrane was washed with 1X TBST (5 times) and chemiluminescence detection was carried out with ECL detection kit (GE health Care, US) using chemiDoc (Amersham TM Imager 680, Japan).

    other:

    Article Title: Villification of the intestinal epithelium is driven by Foxl1 through activation of PDGFRα and BMPs.
    Article Snippet: The slices were then incubated with primary antibodies overnight at 4°C after blocking with blocking buffer (Abcam, ab126587) for 1 hour at room temperature and then with appropriate secondary antibodies at room temperature in the dark for 1 hour.

    Article Title: Villification of the intestinal epithelium is driven by Foxl1 through activation of PDGFRα and BMPs
    Article Snippet: For IF staining, paraffin sections were subjected to pressure cooker antigen retrieval with Tris/EDTA buffer (Abcam, ab93684).



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